Effects of 3,4-oxo-isopropylidene shikimic acid on the inflammatory reaction in rats with tMCAO
Sun Wenyan Department of Pharmacology of Chinese Materia Medica
Beijing University of Chinese Medicine
China
Abstract:
AIM 3,4-oxo-isopropylidene-shikimic acid (ISA) is one of synthetic derivatives of shikimic acid (ISA) which was a component extracted from Illicium verum Hook.f.. It is proved that inflammatory reaction plays an important role in ischemic damage. In this study, effects of ISA on blood brain barrier (BBB) damage, cerebral edema and leukocyte infiltrate in rats with transient middle cerebral artery occlusion (tMCAO) were investigated. METHODS The permeability test of Evans blue (EB) was determined after ischemia 3 hours and reperfusion 3 hours in rats with tMCAO. The content of water and the activity of myeloperoxidase (MPO) in brain as well as immunohistochemistry (IHC) staining of matrix metalloproteinase-9 (MMP-9) were assayed at 21 hours of reperfusion. RESULTS After tMCAO, the cerebral microvessels can be permeated through by Evans blue and IgG, and MMP-9 expressed on them at in quantities. The content of water and the activity of MPO in brain increased obviously. ISA can observably decrease the content of water and Evans blue, reduce the expression of MMP-9 and the activity of MPO (P<0.05 or 0.01, compared with the vehicle group). CONCLUSION ISA can relieve the inflammatory reaction leaded to by cerebral ischemia and reperfusion.
